human lung cancer cell line a427  (DSMZ)

Journal: Pharmaceuticals

Article Title: Self-Assembled Supramolecular Nanoparticles Improve the Cytotoxic Efficacy of CK2 Inhibitor THN7

doi: 10.3390/ph11010010

Figure Lengend Snippet: Cytotoxic activity of THN7-loaded α-cyclodextrin nanoparticles on A-427 lung cancer cells. C4: C 4 H 9 ; C6: C 6 H 13 ; C8: C 8 H 17 . (+) loaded cyclodextrins; (−) empty cyclodextrins. Viability of A427 cells were determined using an MTT assay. The viability of cells treated with 1% of the appropriate solvent was set 100%. Staurosporine served as a control for the correctly performance of the MTT assay using A427 cells. In all experiments THN7 was used at a final concentration of 6 μM.

Article Snippet: The nanoparticles were tested on the human lung cancer cell line A427, which was obtained from the German Collection of Microorganisms and Cell Culture (DSMZ, Braunschweig, Germany).

Techniques: Activity Assay, MTT Assay, Solvent, Control, Concentration Assay

Journal: PLoS ONE

Article Title: Cancer-Type Regulation of MIG-6 Expression by Inhibitors of Methylation and Histone Deacetylation

doi: 10.1371/journal.pone.0038955

Figure Lengend Snippet: (A–C) Different lengths of the MIG-6 promoter regulatory region were inserted into the pGL3 vector. The luciferase reporter assay was performed in A427 lung cancer cells transiently transfected with pGL3-Basic or the indicated reporter carrying MIG-6 promoter element. The cells were then treated with or without 5-aza-dC or TSA. The pU6B- Renilla reporter was co-transfected for normalization. Each assay was performed in triplicate. The error bars represent standard deviation. (D) Schematic representation of the TSA-response element in the MIG-6 promoter regulatory region. The arrow indicates the transcription starting site; the red box indicates exon 1. Shaded in green is the 50-bp element in exon 1 that is most likely responsible for TSA response in lung cancer cells, which we designated as the minimal TSA-response element.

Article Snippet: The human lung cancer cell lines A427, NCI-H292, NCI-H2122, NCI-H596, and SK-MES-1 were obtained from American Type Culture Collection (Manassas, VA).

Techniques: Plasmid Preparation, Luciferase, Reporter Assay, Transfection, Standard Deviation

Journal: PLoS ONE

Article Title: Cancer-Type Regulation of MIG-6 Expression by Inhibitors of Methylation and Histone Deacetylation

doi: 10.1371/journal.pone.0038955

Figure Lengend Snippet: (A) The sequences of P(−76/+50) that contain the MIG-6 promoter and the minimal TSA-response element are shown. For each mutant reporter construct (m3-m11), the underlined sequence was mutated to GAATTC. (B and C) A luciferase reporter assay was performed in A427 lung cancer cells by transiently transfecting the indicated reporter plasmid with or without TSA treatment. The error bars represent standard deviation and all assays were performed in triplicate.

Article Snippet: The human lung cancer cell lines A427, NCI-H292, NCI-H2122, NCI-H596, and SK-MES-1 were obtained from American Type Culture Collection (Manassas, VA).

Techniques: Mutagenesis, Construct, Sequencing, Luciferase, Reporter Assay, Plasmid Preparation, Standard Deviation

Journal: PLoS ONE

Article Title: Cancer-Type Regulation of MIG-6 Expression by Inhibitors of Methylation and Histone Deacetylation

doi: 10.1371/journal.pone.0038955

Figure Lengend Snippet: Microarray analyses were performed on RNA samples from A427 lung cancer cells and M14 melanoma cells treated with or without 5-aza-dC (10 µM) and/or TSA (1 µM). The heat maps show (A) the genes whose expression pattern was similar to that of MIG-6 , and (B) the genes whose expression was down-regulated by the treatment, in contrast to MIG-6 expression. The MIG-6 gene is indicated with an asterisk and is shown as the alternative symbol, ERRFI1 .

Article Snippet: The human lung cancer cell lines A427, NCI-H292, NCI-H2122, NCI-H596, and SK-MES-1 were obtained from American Type Culture Collection (Manassas, VA).

Techniques: Microarray, Expressing

Journal: PLoS ONE

Article Title: Cancer-Type Regulation of MIG-6 Expression by Inhibitors of Methylation and Histone Deacetylation

doi: 10.1371/journal.pone.0038955

Figure Lengend Snippet: The expression of EGR1 was determined by RT-PCR. (A) TSA induced EGR1 expression in the NCI-H226, NCI-H522, NCI-H596, and A427 lung cancer cell lines. (B) 5-Aza-dC induced EGR1 expression in the M14, MALME-3M, SK-2, SK-MEL-28, and UACC-257 melanoma cell lines. GAPDH expression was used as an internal control (see ).

Article Snippet: The human lung cancer cell lines A427, NCI-H292, NCI-H2122, NCI-H596, and SK-MES-1 were obtained from American Type Culture Collection (Manassas, VA).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Control